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DEPARTMENT OF MOLECULAR PLANT PHYSIOLOGY Print option in slimbox / lytebox? (info)
Friday, 08 December 2017 12:51

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Miecznikowa 1, 02-096 Warszawa

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under construction)

Director:
prof. dr hab. Elżbieta Romanowska
phone: (4822) 55 43 916
fax: (4822) 55 43 910
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Staff:
dr Anna Drożak
phone: (4822) 55 43 918
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dr Tomasz Krupnik
phone: (4822) 55 43 912
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dr hab. Eugeniusz Parys prof. UW
(Professor Emeritus)
phone: (4822) 55 43 914
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dr Magdalena Rudnicka
phone: (4822) 55 43 920
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mgr Ewa Wacławczyk-Lach
phone: (4822) 55 43 919

dr Wioleta Wasilewska-Dębowska
phone: (4822) 55 43 918
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dr hab. Maksymilian Zienkiewicz
phone: (4822) 55 43 912
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PhD students:
mgr Ilona Bacławska
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mgr Alicja Buczyńska
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Mgr Paweł Rogowski
phone: (4822) 55 43 920
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Research profile:
Physiological, biochemical and molecular aspects of regulation photosynthesis, respiration and photorespiration in C3, and C4 plants during stress.

Our research aim is the acclimation of the photosynthetic protein complexes in the thylakoid membranes to adverse environmental conditions, and relationships between photosynthesis and respiration. We have recently started a project for structural and functional characterization of mesophyll and bundle sheath complexes (most attention has been put on PSI and PSII complex) in chloroplasts of C4 plants representing three metabolic subtypes. We investigated in parallel metabolite content, redox status and ATP/ADP level in various stress conditions (light intensity and quality, heavy metals,) in chloroplasts and mitochondria. Also supramolecular organization of the thylakoid membrane complexes during acclimation to light intensity of plants with different structure of antenna (C4 plants and extermophilic red alga) is investigated (prof. E. Romanowska project).

We are also interested in:

  • The mechanisms of the photodamage and repair of PSII, and D1 protein turnover in C4 plant chloroplasts. The regulation of thylakoid protein phosphorylation.
  • The expression of the chloroplast genes encoding the various thylakoid proteins when plants are subjected to different irradiances or modulated redox state.
  • Development of self-organised catalyst using highly robust PSI particles from extremophilic red alga C. merolae for sustainable H2 production (J. Olmos)
  • Development of stable transformation system in extremophilic unicellular red alga Cyanidioschyzon merolae 10D (dr M. Zienkiewicz project).

Main publications from 2006:

  1. Parys E., Jastrzębski H. (2006) Light-enhanced dark respiration in leaves, isolated cells and protoplasts of various types of C4 plants. J. Plant Physiol. 163, 638-647.
  2. Romanowska E., Drożak A., Pokorska B., Shiell B. J., Michalski W. (2006) Organization and activity of photosystems in the mesophyll and bundle sheath chloroplasts of maize. J. Plant Physiol. 163, 607-618.
  3. Romanowsk E, Drożak A, Wróblewska B., Siedlecka M. (2006) High light intensity protects photosynthetic apparatus of pea plants against exposure to lead. Plant Physiol. Biochem. 44, 387-394.
  4. Ciosek P., Pokorska B., Romanowska E., Wróblewski W. (2006) Potentiometric electronic tongue for the recognition of growth conditions of plants. Electroanalysis 18, 1266-1272.
  5. Drożak A. , Romanowska E. (2006) Acclimation of mesophyll and bundle sheath chloroplasts of maize to different irradiances during growth. BBA-Bioenergetics 1757, 1539-1546.
  6. Romanowska E., Drożak A. (2006) Comparison of photochemical activities in mesophyll and bundle sheath chloroplasts of C4 subtypes growing in moderate light. Acta Biochimica Polonica 53, 709-719.
  7. Zienkiewicz M., Kern-Zdanowicz I., Golembiewski M., Żylińska J., Mieczkowski P., Gniadkowski M., Bardowski J., Cegłowski P. (2007) Mosaic Structure of p1658/97, a 125-Kilobase Plasmid Harboring an Active Amplicon with the Extended-Spectrum ß-Lactamase Gene blaSHV-5. Antimicrobial Agents and Chemotherapy, 51, 1164-1171.
  8. Pokorska B., Romanowska E. (2007) Photoinhibition and D1 protein degradation in mesophyll and agranal bundle sheath thylakoids of maize. Functional Plany Biology 34, 844-852
  9. Golembiewski J., Kern-Zdanowicz I., Zienkiewicz M., Adamczyk M., Żylińska J., Baraniak A., Gniadkowski M., Bardowski J., Cegłowski P. (2007) Complete nucleotide sequence of the pCTX-M3 plasmid and its involvement in spread of the extended-spectrum {beta}-lactamase (ESBL) gene blaCTX-M-3. Antimicrobial Agents and Chemotherapy 2007 51:3789-3795.
  10. Parys E., Jastrzębski H. (2008) Mitochondria from leaf mesophyll cells of C4 plants are deficient in the H protein of glycine decarboxylase complex. J. Plant Physiol.165, 1061-1069.
  11. Romanowska E., Wróblewska B., Drozak A., Zienkiewicz M., Siedlecka M. (2008) Effect of Pb ions on superoxide dismutase and catalase activities in leaves of pea plants grown in high and low irradiance Biologia Plantarum 52: 80-86.
  12. Romanowska E., Powikrowska M., Zienkiewicz M., Drożak A., Pokorska B. (2008) High light induced accumulation of two isoforms of the CF1 α-subunit in mesophyll and bundle sheath chloroplasts of C4 plants. Acta Biochimica Polonica 55: 175-182.
  13. Romanowska E., Kargul J., Powikrowska M., Finazzi G., Nield J., Drożak A., Pokorska B.(2008) Structural organization of photosynthetic apparatus in agranal chloroplasts of maize. J. Biol. Chem. 283, 26037-96.
  14. Moscicka K.B., Krupnik T., Boekema E.J., Lolkema J.S., (2009) Projection Structure by Single-Particle Electron Microscopy of Secondary Transport Proteins GltT, CitS, and GltS., Biochemistry 48, 6618–6623
  15. Pokorska B, Zienkiewicz M, Powikrowska M, Drozak A i Romanowska E (2009) Differential turnover of the photosystem II reaction centre D1 protein in mesophyll and bundle sheath chloroplasts of maize. BBA 1787,1161-1169.
  16. Krupnik T., Dobrowolski A., Lolkema J.S. (2011) Cross-linking of Dimeric CitS and GltS Transport Proteins. Mol Membr Biol, 28:243-253.
  17. Krupnik T, Sobczak-Elbourne I, Lolkema J.S. (2011) Turnover and accessibility of a reentrant loop of the Na(+)-glutamate transporter GltS are modulated by the central cytoplasmic loop. Mol Membr Biol 28: 462-72.
  18. Bienert MD; Siegmund SEG; Drozak A; Trombik T; Bultreys A; Baldwin IT; Boutry M 2012, A pleiotropic drug resistance transporter in Nicotiana tabacum is involved in defense against the herbivore Manduca sexta. The Plant Journal, 72, 745-757.
  19. Fristedt R, Wasilewska W., Romanowska E, Vener A.V (2012) Differential phosphorylation of thylakoid proteins in mesophyll and bundle sheath chloroplasts from maize plants grown under low or high light. Proteomics,12:1-10. http://dx.doi.org/10.1002/pmic.201200196
  20. Romanowska E., Wasilewska w., Fristedt R., Vener A. (2012) Phosphorylation of PSII proteins in maize thylakoids in the presence of Pb ions. J Plant. Physiol. 169 , 345– 352.
  21. Zienkiewicz M., Ferenc A., Wasilewska W., Romanowska E. (2012) High light stimulates Deg1-dependent cleavage of the minorLHCII antenna proteins CP26 and CP29 and the PsbS protein in Arabidopsis thaliana. Planta, 235:279-288.
  22. Gawrońska K., Romanowska E., Miszalski Z., Niewiadomska E. (2013) Limitation of C3–CAM shift in the common ice plant under high irradiance. Journal of Plant Physiology, 170, 129-135. http://dx.doi.org/10.1016/j.jplph.2012.09.019
  23. Krupnik T, Kotabova E, van Bezouwen LS, Mazur R, Garstka M, Nixon PJ, Barber J, Kana R, Boekema EJ, Kargul J (2013) A reaction centre-dependent photoprotection mechanism in a highly robust photosystem II from an extremophilic red alga Cyanidioschyzon merolae. (2013) J. Biol. Chem., 288, 23529-23542.
  24. Kutyła-Olesiuk A., Ciosek P., Romanowska E., Wróblewski W. (2013) Effect of lead accumulation in maize leaves on their chemical images created by flow-through electronic tongue. Talanta 103, 179-185. http://dx.doi.org/10.1016/j.talanta.2012.10.029
  25. Zienkiewicz M., Kokoszka N., Bacławska I., Drożak A., Romanowska E.(2013) Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plant Physiology and Biochemistry http://dx.doi.org/10.1016/j.plaphy.2013.02.028
  26. Zienkiewicz M., Kern-Zdanowicz I., Carattoli A., Gniadkowski M., Cegłowski P. (2013) Tandem multiplication of the IS26-flanked amplicon with the blaSHV-5 gene within plasmid p1658/97. FEMS Microbiology Letters 341(1):27-36.
  27. Parys E, Wasilewska W, Siedlecka M, Zienkiewicz M, Drożak A, Romanowska E. (2014) Metabolic responses to lead of metallicolous and nonmetallicolous populations of Armeria maritima. Archives of Environmental Contamination and Toxicology 67: 565-577. DOI 10.1007/s00244-014-0057-z
  28. Wasilewska W, Drożak A, Bacławska I, Kąkol K, Romanowska E. (2015) Lead induced changes in phosphorylation of PSII proteins in low light grown pea plants. Biometals 28: 151-162. DOI 10.1007/s10534-014-9811-y
  29. Romanowska E, Parys E, Wasilewska W. 2015. „Działanie metali ciężkich na wybrane procesy metaboliczne” w: „Ekotoksykologia: rośliny, gleby, metale”- monografia, Wierzbicka M. (red.), wyd. UW, 60-82.
  30. Zienkiewicz M, Drożak A, Wasilewska W, Bacławska I, Przedpełska-Wąsowicz E, Romanowska E (2015) The short-term response of Arabidopsis thaliana (C3) and Zea mays (C4) chloroplasts to red and far red light. Planta, 242:1479–1493. DOI:10.1007/s00425-015-2392-3
  31. Zienkiewicz M., Krupnik T., Drożak A., Golke A., Romanowska E.(2015) Chloramphenicol acetyltransferase—a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae. Protoplasma DOI 10.1007/s00709-015-0936-9
  32. Zienkiewicz M., Krupnik T., Drożak A., Golke A., Romanowska E. (2016) Transformation of the Cyanidioschyzon merolae chloroplast genome: prospects for understanding chloroplast function in extreme environments.. Plant Mol Biol doi:10.1007/s11103-016-0554-8
  33. Romanowska E., Buczyńska A., Wasilewska W., Krupnik T., Drożak A., Rogowski P., Parys E., Zienkiewicz M. (2017) Differences in photosynthetic responses of NADP-ME type C4 species to high light. Planta, 245: 641-657. DOI :10.1007/s00425-016-2632-1
  34. Zienkiewicz M., Krupnik T., Drożak A., Wasilewska W., Golke A., Romanowska E.(2007) Deletion of psbQ' gene in Cyanidioschyzon merolae reveals the function of extrinsic PsbQ' in PSII. Plant Mol. Biol. DOI10. 1007/s11103-017-0685-6