Flow Cytometry Laboratory
The Flow Cytometry Laboratory offers high-quality analyses of animal, plant, and microbial cells using multiparameter flow cytometry.
The Laboratory can assess up to 9 parameters simultaneously and allows the measurement of large numbers of individual cells or biological “particles” in a short time. The analyses performed in the Laboratory also enable the detection of very rare cell populations together with their quantitative and qualitative assessment. The available services include cell sorting into up to four populations at the same time for further analyses, immunophenotyping, and analysis of the functional state of cells.
Thanks to many years of experience, the Laboratory also offers specialist support in planning and analysing studies carried out by clients. Educational support is also provided through individual and group training.
The Laboratory provides services to research institutions, research and development organisations, and commercial partners.
The following excitation lasers and fluorescence detection channels are available:
Violet laser, 405 nm
Sorting: 450/40, 530/30
Analysis: 448/45, 528/45
Blue laser, 488 nm
Sorting: 530/30, 585/42, 616/23, 695/40, 780/60
Analysis: 488/15, 527/32, 585/42, 700/54, 783/56
Red laser, 640 nm
Sorting: 660/20, 780/60
Analysis: 660/10, 783/56
Scope of Services
Available Applications
- Cell population sorting
- Multiparameter analyses:
- Immunophenotyping
- Apoptosis and cell viability
- Cell cycle and cell proliferation
- Detection of intracellular proteins
- Cytokine analysis using Cytometric Bead Array (CBA) or antibodies
- Cytotoxicity testing
- Assessment of transfected cells
- Microbiological analyses of bacteria, algae, protozoa, and yeast:
- Identification based on autofluorescence or DNA staining
- Assessment of viability and cell numbers
Expert Support
- Individual training:
- performing selected analyses from the Laboratory’s standard offer
- performing analyses according to individual requirements
- Analysis of cytometric data from .fcs files using:
- BD FACSDiva™, BD FACSuite™, FCAP Array
- CytoExpert, Kaluza
- Consultations:
- support in planning experiments using flow cytometry
- design of multicolour panels, including fluorochrome selection, selection of appropriate controls, and design of antibody titration experiments
Equipment
Cell Sorters
- FACSAria, BD Biosciences
Analysers
- FACSVerse, BD Biosciences
- FACSCalibur, BD Biosciences
